The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells.
Two distinct types of T-cell antigen receptors have been identified: the alpha/beta heterodimer found on functional helper and cytotoxic T cells, and the gamma/delta heterodimer. The latter is first detected approximately 2 days before the appearance of cell-surface alpha/beta heterodimer during T-cell ontogeny.
Haskins K et al. The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J Exp Med157:1149-69 (1983).
Read more (PubMed: 6601175) »
Marrack P et al. The major histocompatibility complex-restricted antigen receptor on T cells. IV. An antiidiotypic antibody predicts both antigen and I-specificity. J Exp Med158:1635-46 (1983).
Read more (PubMed: 6195288) »