Anti-T-bet / Tbx21抗体[4B10] (ab91109)

概述

  • 产品名称Anti-T-bet / Tbx21抗体[4B10]
    参阅全部 T-bet / Tbx21 一抗
  • 描述
    小鼠单克隆抗体[4B10] to T-bet / Tbx21
  • 经测试应用适用于: IHC-P, WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    E. coli protein

  • 阳性对照
    • Th1 polarized Mouse CD4+ T cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

性能

应用

Our Abpromise guarantee covers the use of ab91109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
  • 组织特异性T-cell specific.
  • 疾病相关Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
  • 序列相似性Contains 1 T-box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • T bet antibody
    • T box 21 antibody
    • T box expressed in T cells antibody
    • T box protein 21 antibody
    • T box transcription factor TBX21 antibody
    • T cell specific T box transcription factor antibody
    • T cell specific T box transcription factor T bet antibody
    • T PET antibody
    • T-box protein 21 antibody
    • T-box transcription factor TBX21 antibody
    • T-cell-specific T-box transcription factor T-bet antibody
    • TBET antibody
    • TBLYM antibody
    • TBX 21 antibody
    • Tbx21 antibody
    • TBX21_HUMAN antibody
    • TPET antibody
    • Transcription factor TBLYM antibody
    see all

Anti-T-bet / Tbx21 antibody [4B10] 图像

  • All lanes : Anti-T-bet / Tbx21 antibody [4B10] (ab91109) at 2 µg/ml

    Lane 1 : Lysates of CD4+ T cells (control)
    Lane 2 : Lysates of PMA and Ionomycin reactivated CD4+ T cells

    Secondary
    HRP anti-mouse IgG

    Predicted band size : 58 kDa
  • Overlay histogram showing Jurkat cells stained with ab91109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91109, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • IHC image of T-bet / Tbx21 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91109, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-T-bet / Tbx21 antibody [4B10] (ab91109)参考文献

This product has been referenced in:
  • Kanagavelu S  et al. TIR-domain-containing adapter-inducing interferon-ß (TRIF) regulates Th17-mediated intestinal immunopathology in colitis. Mucosal Immunol N/A:N/A (2014). Read more (PubMed: 25073675) »
  • Kelhälä HL  et al. IL-17/Th17 Pathway Is Activated in Acne Lesions. PLoS One 9:e105238 (2014). IHC-P ; Human . Read more (PubMed: 25153527) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (CD3 T cells isolated from mouse gut)
Specification CD3 T cells isolated from mouse gut
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 15 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human gut biopsies)
Loading amount 100 µg
Specification human gut biopsies
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"