Anti-Syntrophin alpha 1抗体(ab11187)

Abreviews (2)Q&A (6)Specific References (4)

概述

  • 产品名称
    Anti-Syntrophin alpha 1抗体
    参阅全部 Syntrophin alpha 1 一抗
  • 描述
    兔多克隆抗体to Syntrophin alpha 1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Rabbit, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 191-206 of Mouse Syntrophin alpha 1.

  • 常规说明
    For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-Fr 1/1000.
ICC/IF 1/200.
WB 1/2000. Predicted molecular weight: 60 kDa.

靶标

  • 功能
    Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate.
  • 组织特异性
    High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • 疾病相关
    Defects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • 序列相似性
    Belongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • 结构域
    The PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • 翻译后修饰
    Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • 细胞定位
    Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Acidic alpha 1 syntrophin antibody
    • Alpha 1 syntrophin antibody
    • Alpha-1-syntrophin antibody
    • dJ1187J4.5 antibody
    • Dystrophin associated protein A1 59kDa acidic component antibody
    • LQT12 antibody
    • OTTHUMP00000030650 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • Syntrophin 1 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

文献

This product has been referenced in:
  • Anderova M  et al. Altered astrocytic swelling in the cortex of a-syntrophin-negative GFAP/EGFP mice. PLoS One 9:e113444 (2014). WB ; Mouse . Read more (PubMed: 25426721) »
  • Koo T  et al. Delivery of AAV2/9-Microdystrophin Genes Incorporating Helix 1 of the Coiled-Coil Motif in the C-Terminal Domain of Dystrophin Improves Muscle Pathology and Restores the Level of a1-Syntrophin and a-Dystrobrevin in Skeletal Muscles of mdx Mice. Hum Gene Ther : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21453126) »

See all 4 Publications for this product

发表研究结果有使用 ab11187？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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8 reviews or Q&A

10
Votes: Highest First

Can I ask you at which address are you going to send them? Just in case:

Thank you for contacting us. I am sorry but I did not receive your email until this morning. This order has been delivered already using the address of the original order (xxxx). The attention line was to Receiving/PO#xxxx. FedEx tracking number is...

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As you proposed, I’ll be glad to try as well your rabbit monoclonal anti-muscle Actin ab109499 Thanks a lot

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you so much I’ve seen a PO # xxxx and xxxx, I don’t know which one is the oldest Thanks

Thank you for your reply.

Neither of these are for the product from December of last year but are much older. If you could find the number for the most recent order of ab11187 it would be very helpful for creating this replacement. Also, i...

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I was using it for western blot only that’s why it took so long for me to realize that it was not good for IF. I could buy a new lot, do you have a different one from GR8082? would you accept at least to make a discount?

Thank you for contacting us. I am very happy to to provide this antibody free of charge to you. I see that the original order for the faulty lot may have been placed in December. Could you please confirm this for me and perhaps provide and order num...

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I used different protocols: from frozen muscle sections   -          Slides at room temperature for 30min -          PBS 15-30min -          Blocking in 10% goat serum for 30min-1h -          syntrophin at 1/100 or 1/200 overnight (in PBS+2% goat...

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Thank you for providing this information.

I am very sorry that this product did not work as expected and wish to apologize for the inconvenience this has caused. In reviewing the protocol details which you have provided I see no reason why t...

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Hi, I used to work with the lot number 79395, old one. But your new alpha1-syntrophin antibody is not good. I don't have the same nice results anymore and I can't work with the same dilutions. So for WB, it is ok but for IF, even at 1:100, the signal i...

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Thank you for contacting us.

I am very sorry to hear that you have been having difficulties when using this recent lot in ICC/IF. Sadly, the lot which you have requested, 79335, is not longer in stock. However, I would be very happy to try...

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Western blot abreview for Anti-Syntrophin alpha 1 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (rat spinal cord cytosolic and membranous extract)
Loading amount
40 µg
Specification
rat spinal cord cytosolic and membranous extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Username

Abcam user community

Verified customer

提交于 Jun 21 2007

Immunocytochemistry/ Immunofluorescence abreview for Anti-Syntrophin alpha 1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (skeletal muscle)
Specification
skeletal muscle
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2%
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abreview image
Username

Dr. Bruno Constantin

Verified customer

提交于 Mar 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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