概述

  • 产品名称Anti-Syntenin抗体
    参阅全部 Syntenin 一抗
  • 描述
    兔多克隆抗体to Syntenin
  • 经测试应用适用于: IHC-P, IHC-FoFr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis
    预测可用于: Monkey, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse Syntenin.

  • 阳性对照
    • HEK 293 cells, human, rat and mouse heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab19903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/1000.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.
  • 组织特异性Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
  • 序列相似性Contains 2 PDZ (DHR) domains.
  • 翻译后修饰Phosphorylated on tyrosine residues.
  • 细胞定位Cell junction > focal adhesion. Cell junction > adherens junction. Cell membrane. Endoplasmic reticulum membrane. Nucleus. Melanosome. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MDA-9 antibody
    • MDA9 antibody
    • Melanoma differentiation-associated protein 9 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 18 antibody
    • Scaffold protein Pbp1 antibody
    • SDCB1_HUMAN antibody
    • SDCBP antibody
    • ST1 antibody
    • SYCL antibody
    • Syndecan binding protein (syntenin) antibody
    • Syndecan binding protein 1 antibody
    • Syndecan-binding protein 1 antibody
    • Syntenin 1 antibody
    • Syntenin-1 antibody
    • TACIP18 antibody
    see all

Anti-Syntenin antibody 图像

  • All lanes : Anti-Syntenin antibody (ab19903) at 1 µg/ml

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Mouse heart lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
    Lane 4 : Rat heart lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    ab19903 detects a band of the expected size (32kDa) in both mouse and rat heart lysate. This band is quenched completely by the addition of the immunizing peptide in the mouse lysate and is partially quenched by the addition of ab20431 in the rat heart lysate.
  • ICC/IF image of ab19903 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab19903, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Image courtesy of Human Protein Atlas
    ab19903 staining Syntenin in human heart tissue. Paraffin-embedded tissue was cut into 4µm sections and incubated with ab19903 (1/100 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab19903 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
  • IHC-FoFr image of Syntenin staining using ab19903 (1:1000) on Mouse cortex (A) and mouse paraventricular nucleus (B). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-Syntenin antibody (ab19903)参考文献

This product has been referenced in:
  • Gangemi R  et al. Mda-9/syntenin is expressed in uveal melanoma and correlates with metastatic progression. PLoS One 7:e29989 (2012). WB, IHC-P ; Human . Read more (PubMed: 22267972) »
  • Lazic D  et al. Human papillomavirus type 8 E6 oncoprotein inhibits transcription of the PDZ protein syntenin-2. J Virol 86:7943-52 (2012). WB ; Human . Read more (PubMed: 22623796) »

See all 3 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

提交于 May 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (meiotic egg extract)
Specification meiotic egg extract
Fixative pre-fixation in formaldehyde and Methanol fixation after extract spindown onto coverslips
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"