Anti-Syntenin 2抗体(ab83537)

概述

  • 产品名称Anti-Syntenin 2抗体
    参阅全部 Syntenin 2 一抗
  • 描述
    兔多克隆抗体to Syntenin 2
  • 经测试应用适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (VAPVTGYSLG VRRAEIKPGV REIHLCKDER GKTGLRLRKV DQGLFVQLVQ) of Human Syntenin 2 (NP_056500; Q9H190-3).

  • 阳性对照
    • Hela cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Syntenin 2 antibody 图像

  • Anti-Syntenin 2 antibody (ab83537) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • ab83537 staining Syntenin 2 in the MCF7, human breast epithelial from Human mammary glands by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde or 100% Methanol, permeabilized with Digitonin and blocked with 1% BSA for 10 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 21°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. DAPI was Diluted 1/1000 and samples mounted in gel.

    See Abreview

Anti-Syntenin 2 antibody (ab83537)参考文献

ab83537 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Various cell types, see image!)
Loading amount 50 µg
Specification Various cell types, see image!
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Prof. Ritva Tikkanen

Verified customer

提交于 Aug 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7, human breast epithelial)
Specification MCF7, human breast epithelial
Fixative Paraformaldehyde
Permeabilization Yes - digitonin
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Prof. Ritva Tikkanen

Verified customer

提交于 Aug 22 2012

ab57063 : DISCOUNT CODE: 100%ABR-000
ab83537: DISCOUNT CODE: 100%ABR-0000

Expiration date: 04 October 2012

I am very pleased to hear you would like to accept our offer and test ab57063 and ab83537in IF and/or IP.Each code will...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"