Anti-Syntenin 2抗体(ab38745)

概述

  • 产品名称Anti-Syntenin 2抗体
    参阅全部 Syntenin 2 一抗
  • 描述
    山羊多克隆抗体to Syntenin 2
  • 特异性This antibody is expected to recognise both human isoforms (NP_056500; NP_536737).
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Syntenin 2 aa 280-292 (C terminal).
    Sequence:

    LLHHTMDHSIPDA

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 6 µg/ml.

靶标

Anti-Syntenin 2 antibody 图像

  • ab38745 staining Syntenin in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated rabbit anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab38745 (4µg/ml) staining of paraffin embedded Human Liver shows staining of cytoplasm in hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-Syntenin 2 antibody (ab38745)参考文献

ab38745 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Mar 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"