RabMAb

Anti-Syntaxin-binding protein 4抗体[EP302Y] (ab40674)

概述

  • 产品名称Anti-Syntaxin-binding protein 4抗体[EP302Y]
    参阅全部 Syntaxin-binding protein 4 一抗
  • 描述
    兔单克隆抗体[EP302Y] to Syntaxin-binding protein 4
  • 特异性This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis.
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of the PDZ domain of Human Synip.

  • 阳性对照
    • Human skeletal lysate and human lung carcinoma tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab40674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/250 - 1/500.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.
    • 序列相似性Contains 1 PDZ (DHR) domain.
      Contains 1 WW domain.
    • 翻译后修饰Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ16496 antibody
      • MGC149829 antibody
      • MGC50337 antibody
      • OTTHUMP00000082667 antibody
      • OTTHUMP00000227400 antibody
      • STX4-interacting protein antibody
      • STXB4_HUMAN antibody
      • Stxbp4 antibody
      • Synip antibody
      • Syntaxin 4 interacting protein antibody
      • Syntaxin 4-interacting protein antibody
      • Syntaxin binding protein 4 antibody
      • Syntaxin-binding protein 4 antibody
      see all

    Anti-Syntaxin-binding protein 4 antibody [EP302Y] 图像

    • ab40674 at a 1:250 dilution staining Synip in human lung carcinoma.
    • Anti-Syntaxin-binding protein 4 antibody [EP302Y] (ab40674) at 1/5000 dilution + human skeletal lysate

      Predicted band size : 61 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Anti-Syntaxin-binding protein 4 antibody [EP302Y] (ab40674)参考文献

    This product has been referenced in:
    • Taniai E  et al. Crosstalk between PTEN/Akt2 and TGFbeta signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model. Cancer Sci 100:813-20 (2009). IHC-P ; Rat . Read more (PubMed: 19309364) »
    • Okada S  et al. Synip phosphorylation is required for insulin-stimulated Glut4 translocation. Biochem Biophys Res Commun 356:102-6 (2007). Read more (PubMed: 17336927) »

    See all 2 Publications for this product

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    The immunogen sequence of anti TRAIL antibody used in this kit corresponds to 114-281 of human TRAIL. It is more likely that it will detect your partial N-terminus (aa114-281) protein.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"