Anti-Syntaxin抗体[STX01 (HPC-1)] (ab3265)

概述

  • 产品名称Anti-Syntaxin抗体[STX01 (HPC-1)]
    参阅全部 Syntaxin 一抗
  • 描述
    小鼠单克隆抗体[STX01 (HPC-1)] to Syntaxin
  • 特异性In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    amino acids 2-335 of Rat Syntaxin

  • 阳性对照
    • IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab3265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
IP 1/200.
ICC/IF 1/50.

靶标

Anti-Syntaxin antibody [STX01 (HPC-1)] 图像

  • All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Spinal Cord (Rat) Tissue Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)参考文献

This product has been referenced in:
  • Yun HJ  et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. Exp Mol Med 45:e36 (2013). Read more (PubMed: 23949442) »
  • Kunzevitzky NJ  et al. Amacrine cell subtypes differ in their intrinsic neurite growth capacity. Invest Ophthalmol Vis Sci 54:7603-13 (2013). IHC ; Rat . Read more (PubMed: 24130183) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. I am sorry to hear that you are having problems with the 3 antibodies ab53723, ab11104 and ab3265. Unfortunately, ab53723 has not been tested for IHC-Frozen, horse and pig samples. Also, ab11104 has not yet been teste...

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Thank you very much for your patience. Product ab3265 is raised against Syntaxin 1 of rat origin. Since there are only 2 amino acids that fall within an extracellular region, the chances of Syntaxin 1 (HPC-1) mapping to this region are quite low...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (cranial cervical ganglion)
Specification cranial cervical ganglion
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH Vector labs H-3301
Permeabilization No
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Abcam user community

Verified customer

提交于 Feb 09 2011

Thank you for getting back to me. The details of the epitope region (extracellular) are provided by the source of this antibody and is shown on the datasheet. Please see the link below. I hope this information helps. Please do not hesitate to c...

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Thank you for your enquiry. I am sorry but the epitope sequence ab3265 remains proprietary. I can however tell you that the epitope resides in the extracellular domain of the protein. I hope this information helps. Please do not hesitate to con...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please le...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"