概述

  • 产品名称Anti-Syntaxin 4抗体
    参阅全部 Syntaxin 4 一抗
  • 描述
    小鼠单克隆抗体to Syntaxin 4
  • 经测试应用适用于: WB, Flow Cyt, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant protein fragment with GST tag, corresponding to amino acids 19 - 120 of Human Syntaxin 4 (NP_004595)

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration. Detection limit for ab77037 is approximately 1ng/ml as a capture antibody.

靶标

  • 功能Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.
  • 组织特异性Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.
  • 序列相似性Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NY REN 31 antigen antibody
    • p35 2 antibody
    • PLACENTAL antibody
    • Renal carcinoma antigen NY REN 31 antibody
    • Renal carcinoma antigen NY-REN-31 antibody
    • Stx4 antibody
    • STX4_HUMAN antibody
    • STX4A antibody
    • Syntaxin 4 antibody
    • syntaxin 4A (placental) antibody
    • Syntaxin 4A antibody
    • Syntaxin-4 antibody
    see all

Anti-Syntaxin 4 antibody 图像

  • Anti-Syntaxin 4 antibody (ab77037) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anit-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Anti-Syntaxin 4 antibody (ab77037) at 1/500 dilution + recombinant protein Syntaxin 4 at 0.1 µg

    Secondary
    Goat anit-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ab77037 tested in recombinant GST tagged protein, with a detection limit of approximately 1 ng/ml
  • ICC/IF image of ab77037 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77037, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HL60 cells stained with ab77037 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77037, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed in 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Syntaxin 4 antibody (ab77037)参考文献

This product has been referenced in:
  • Kioumourtzoglou D  et al. Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism. Mol Cell Biol 34:1271-9 (2014). Read more (PubMed: 24469400) »
  • Cook SA  et al. Abnormal myocardial insulin signalling in type 2 diabetes and left-ventricular dysfunction. Eur Heart J 31:100-11 (2010). Read more (PubMed: 19797329) »

See all 2 Publications for this product

Product Wall


Unfortunately, I cannot provide any images to back this up as the supplier of this antibody doesn't approve this. However, I would like to assure you that this antibody has been tested in very successfully in Raw 264.7 (Mouse leukaemic monocyte ma...

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