Anti-Syntaxin 1a抗体(ab13262)

概述

  • 产品名称Anti-Syntaxin 1a抗体
    参阅全部 Syntaxin 1a 一抗
  • 描述
    兔多克隆抗体to Syntaxin 1a
  • 特异性Unfortunately we can not exlude crossreactivity with the very homologue protein Syntaxin 1b as the alignement score of the immunogen is relatively high with mouse Syntaxin 1b.
  • 经测试应用适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chicken, Human, Zebrafish
  • 免疫原

    Synthetic peptide:

    MKDRTQELRTAKDSDDDDD

    conjugated to KLH, corresponding to amino acids 1-19 of Human Syntaxin.

  • 阳性对照
    • Rat or Mouse Brain Tissue Extract.

性能

应用

Our Abpromise guarantee covers the use of ab13262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35-36 kDa (predicted molecular weight: 34.6 kDa).
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • 组织特异性Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
  • 疾病相关Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 细胞定位Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HPC 1 antibody
    • Neuron specific antigen HPC1 antibody
    • Neuron-specific antigen HPC-1 antibody
    • OTTHUMP00000174615 antibody
    • OTTHUMP00000174616 antibody
    • OTTHUMP00000174617 antibody
    • OTTHUMP00000174618 antibody
    • P35-1 antibody
    • STX1 antibody
    • STX1A antibody
    • STX1A_HUMAN antibody
    • SYN1A antibody
    • Syntaxin 1A (brain) antibody
    • Syntaxin 1A brain antibody
    • Syntaxin-1A antibody
    see all

Anti-Syntaxin 1a antibody 图像

  • All lanes : Anti-Syntaxin 1a antibody (ab13262) at 1/2000 dilution

    Lane 1 : Chicken brain tissue lysate
    Lane 2 : Chicken DRG tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP-conjugated donkey anti-rabbit IgG at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size : 34.6 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Syntaxin 1a antibody (ab13262)参考文献

ab13262 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (Extra PAGE ONE 5-20%)
Sample Chicken Tissue lysate - whole (Brain and DRG)
Specification Brain and DRG
Blocking step Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
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Verified customer

提交于 Feb 13 2015

Thank you very much for your patience while I was in contact with the laboratory. Please find here the alignment or comments to our other Syntaxin-1a antibodies. It was in the end more difficult than expected to find an antibody which I could recommend...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"