Anti-Syntaxin 1a抗体(ab13262)

概述

  • 产品名称
    Anti-Syntaxin 1a抗体
    参阅全部 Syntaxin 1a 一抗
  • 描述
    兔多克隆抗体to Syntaxin 1a
  • 特异性
    Unfortunately we can not exlude crossreactivity with the very homologue protein Syntaxin 1b as the alignement score of the immunogen is relatively high with mouse Syntaxin 1b.
  • 经测试应用
    适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chicken, Human, Zebrafish
  • 免疫原

    Synthetic peptide:

    MKDRTQELRTAKDSDDDDD

    conjugated to KLH, corresponding to amino acids 1-19 of Human Syntaxin.

  • 阳性对照
    • Rat or Mouse Brain Tissue Extract.

性能

应用

Our Abpromise guarantee covers the use of ab13262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35-36 kDa (predicted molecular weight: 34.6 kDa).
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • 组织特异性
    Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
  • 疾病相关
    Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 细胞定位
    Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HPC 1 antibody
    • Neuron specific antigen HPC1 antibody
    • Neuron-specific antigen HPC-1 antibody
    • OTTHUMP00000174615 antibody
    • OTTHUMP00000174616 antibody
    • OTTHUMP00000174617 antibody
    • OTTHUMP00000174618 antibody
    • P35-1 antibody
    • STX1 antibody
    • STX1A antibody
    • STX1A_HUMAN antibody
    • SYN1A antibody
    • Syntaxin 1A (brain) antibody
    • Syntaxin 1A brain antibody
    • Syntaxin-1A antibody
    see all

图片

  • All lanes : Anti-Syntaxin 1a antibody (ab13262) at 1/2000 dilution

    Lane 1 : Chicken brain tissue lysate
    Lane 2 : Chicken DRG tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP-conjugated donkey anti-rabbit IgG at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size : 34.6 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab13262 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (Extra PAGE ONE 5-20%)
Sample
Chicken Tissue lysate - whole (Brain and DRG)
Specification
Brain and DRG
Blocking step
Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Feb 13 2015

Thank you very much for your patience while I was in contact with the laboratory. Please find here the alignment or comments to our other Syntaxin-1a antibodies. It was in the end more difficult than expected to find an antibody which I could recommend...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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