Anti-Syntaxin 1a抗体(ab103179)

概述

  • 产品名称Anti-Syntaxin 1a抗体
    参阅全部 Syntaxin 1a 一抗
  • 描述
    兔多克隆抗体to Syntaxin 1a
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 36-65 of Human Syntaxin 1a.

  • 阳性对照
    • 293 cell line lysates; Human skeletal muscle tissue.

性能

应用

Our Abpromise guarantee covers the use of ab103179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • 组织特异性Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
  • 疾病相关Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 细胞定位Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HPC 1 antibody
    • Neuron specific antigen HPC1 antibody
    • Neuron-specific antigen HPC-1 antibody
    • OTTHUMP00000174615 antibody
    • OTTHUMP00000174616 antibody
    • OTTHUMP00000174617 antibody
    • OTTHUMP00000174618 antibody
    • P35-1 antibody
    • STX1 antibody
    • STX1A antibody
    • STX1A_HUMAN antibody
    • SYN1A antibody
    • Syntaxin 1A (brain) antibody
    • Syntaxin 1A brain antibody
    • Syntaxin-1A antibody
    see all

Anti-Syntaxin 1a antibody 图像

  • Anti-Syntaxin 1a antibody (ab103179) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 33 kDa
  • ab103179 at 1/50 dilution, staining Syntaxin 1a in Human skeletal muscle by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-Syntaxin 1a antibody (ab103179)参考文献

ab103179 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Purified protein (primary cadiocyte)
Loading amount 30 µg
Specification primary cadiocyte
Gel Running Conditions Reduced Denaturing (gel 12%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Meng Liu

Verified customer

提交于 Feb 28 2012

Thank you very much for your interest in ab103179. The immunogenic region (amino acids 36 - 65 of Human Syntaxin 1a) shows 100% homology with the Mouse Syntaxin 1a. Because we carry over 70,000 products, it isn't feasible for us to keep small sampl...

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Thank you very much for your patience while I was in contact with the laboratory. Please find here the alignment or comments to our other Syntaxin-1a antibodies. It was in the end more difficult than expected to find an antibody which I could recommend...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"