antigen sequence, corresponding to amino acids 694-781 (FMAFSNSSIS SEQKPDDKYD ALKEEASPVP LTSNVGSTVK GGQNSTAAST KYDVFRQLSL EGSGLGVEDL KDNTPSGKSD DDFADFHS) of Human SYNRG.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins.
Contains 1 EH domain.
The DFXDF motifs mediate the interaction with gamma-appendage subunits AP1G1 and AP1G2.
Cytoplasm. Golgi apparatus > trans-Golgi network membrane. Associates with membranes via the adapter protein complex AP-1. Colocalizes with AP1G1.
Information by UniProt
- AP1 subunit gamma-binding protein 1 antibody
- AP1GBP1 antibody
- Gamma-synergin antibody
Immunocytochemistry/ Immunofluorescence - Anti-SYNRG antibody (ab121760)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121760 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNRG antibody (ab121760)
ab121760 at 1/35 dilution staining SYNRG in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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