概述

  • 产品名称Anti-Synphilin 1抗体
    参阅全部 Synphilin 1 一抗
  • 描述
    兔多克隆抗体to Synphilin 1
  • 特异性Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet.
  • 经测试应用适用于: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    CASKGKNKAA

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 911-919 of Human Synphilin 1.

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa
  • 常规说明This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson’s diseased human brain. It is also useful for western blot, identifying a putative Synphilin-1 band.

性能

应用

Our Abpromise guarantee covers the use of ab6179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/1000.
ICC/IF 1/200.

靶标

  • 功能Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
  • 组织特异性Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
  • 疾病相关Parkinson disease
  • 序列相似性Contains 6 ANK repeats.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.
  • 细胞定位Cytoplasm. Detected in cytoplasmic inclusion bodies, together with SNCA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha synuclein interacting protein antibody
    • Alpha-synuclein-interacting protein antibody
    • MGC39814 antibody
    • SNCAIP antibody
    • SNCAP_HUMAN antibody
    • Sph1 antibody
    • Synphilin-1 antibody
    • Synphilin1 antibody
    • Synuclein alpha interacting protein (synphilin) antibody
    • Synuclein alpha interacting protein antibody
    • SYPH 1 antibody
    see all

Anti-Synphilin 1 antibody 图像

  • ICC/IF image of ab6179 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6179 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Synphilin 1 antibody (ab6179)参考文献

This product has been referenced in:

See all 2 Publications for this product

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Thank you for your enquiry and for filling out our technical questionnaire so comprehensively. I am sorry to hear that you have been having difficulties with this antibody. Your data is very conclusive and seems to suggest that this antibody cross r...

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Synphilin-1 cannot be detected in whole brain homogenates without immunoprecipitation first. When the antibody is used to immunoprecipitate material from whole rat brain, the major band is at approximately 90kDa and faint bands at approximately 60 a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"