重组Anti-SynGAP抗体[EPR2883Y] (ab77235)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2883Y] to SynGAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SynGAP抗体[EPR2883Y]
参阅全部 SynGAP 一抗 -
描述
兔单克隆抗体[EPR2883Y] to SynGAP -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SynGAP (C terminal). The exact sequence is proprietary.
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阳性对照
- Fetal brain lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2883Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77235于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 147 kDa.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 147 kDa. |
靶标
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功能
Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits. -
疾病相关
Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients. -
序列相似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain. -
翻译后修饰
Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. - Information by UniProt
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数据库链接
- Entrez Gene: 8831 Human
- Entrez Gene: 240057 Mouse
- Entrez Gene: 192117 Rat
- Omim: 603384 Human
- SwissProt: Q96PV0 Human
- SwissProt: Q9QUH6 Rat
- Unigene: 586264 Human
- Unigene: 9908 Rat
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别名
- DKFZp761G1421 antibody
- KIAA1938 antibody
- MRD5 antibody
see all
图片
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All lanes : Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution
Lane 1 : Hippocampus (Rat) Tissue Lysate
Lane 2 : Cortex (Rat) Tissue Lysate
Lane 3 : Hippocampus (Mouse) Tissue Lysate
Lane 4 : Cortex (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 147 kDa
Observed band size: 130,148 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab77235 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
The doublet observed in the Western blot image is consistent with the literature (PMID:9581761).
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Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution + fetal brain lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 147 kDa
Additional bands at: 50 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab77235 被引用在 7 文献中.
- Kilinc M et al. Endogenous Syngap1 alpha splice forms promote cognitive function and seizure protection. Elife 11:N/A (2022). PubMed: 35394425
- Llamosas N et al. SYNGAP1 Controls the Maturation of Dendrites, Synaptic Function, and Network Activity in Developing Human Neurons. J Neurosci 40:7980-7994 (2020). PubMed: 32887745
- Mastro TL et al. A sex difference in the response of the rodent postsynaptic density to synGAP haploinsufficiency. Elife 9:N/A (2020). PubMed: 31939740
- Dosemeci A et al. Postsynaptic densities fragment into subcomplexes upon sonication. Mol Brain 12:72 (2019). PubMed: 31439005
- Yokoi S et al. 3'UTR Length-Dependent Control of SynGAP Isoform a2 mRNA by FUS and ELAV-like Proteins Promotes Dendritic Spine Maturation and Cognitive Function. Cell Rep 20:3071-3084 (2017). WB, ICC, IHC . PubMed: 28954225
- Yang Y et al. Camkii-mediated phosphorylation regulates distributions of Syngap-a1 and -a2 at the postsynaptic density. PLoS One 8:e71795 (2013). Electron Microscopy, WB ; Rat . PubMed: 23967245
- McMahon AC et al. SynGAP isoforms exert opposing effects on synaptic strength. Nat Commun 3:900 (2012). WB, IF ; Mouse . PubMed: 22692543