概述

  • 产品名称
  • 描述
    兔多克隆抗体to SynGAP
  • 经测试应用
    适用于: IP, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Fusion protein corresponding to Rat SynGAP aa 947-1167.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 148 kDa.

靶标

  • 功能
    Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
  • 疾病相关
    Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
  • 序列相似性
    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
  • 翻译后修饰
    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp761G1421 antibody
    • KIAA1938 antibody
    • MRD5 antibody
    • Neuronal RasGAP antibody
    • OTTHUMP00000064825 antibody
    • p135 SynGAP antibody
    • Ras GTPase activating protein SynGAP antibody
    • Ras GTPase-activating protein SynGAP antibody
    • RASA 1 antibody
    • RASA 5 antibody
    • RASA1 antibody
    • RASA5 antibody
    • SYGP1_HUMAN antibody
    • Synaptic Ras GAP 1 antibody
    • Synaptic Ras GTPase activating protein 1 antibody
    • Synaptic Ras GTPase activating protein 1 homolog antibody
    • Synaptic Ras GTPase activating protein 135kDa antibody
    • Synaptic Ras GTPase activating protein antibody
    • Synaptic Ras GTPase-activating protein 1 antibody
    • Synaptic Ras-GAP 1 antibody
    • SYNGAP 1 antibody
    • SYNGAP1 antibody
    see all

Anti-SynGAP antibody 图像

  • Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

  • Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

Anti-SynGAP antibody (ab3344)参考文献

This product has been referenced in:
  • Bayés A  et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). Read more (PubMed: 23071613) »
  • Yang Y  et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . Read more (PubMed: 21736925) »

See all 2 Publications for this product

Product Wall

Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Various ovarian cancer cell lines)
Specification
Various ovarian cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 11 2015

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