概述

  • 产品名称Anti-Syndecan 4抗体
    参阅全部 Syndecan 4 一抗
  • 描述
    兔多克隆抗体to Syndecan 4
  • 经测试应用适用于: ICC/IF, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 184 of human Syndecan 4 (Intracellular domain).

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab24511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml. PubMed: 22513363
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 5 - 10 µg/ml. PubMed: 22048770
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).Can be blocked with Syndecan 4 peptide (ab52121).

靶标

  • 功能Cell surface proteoglycan that bears heparan sulfate.
  • 组织特异性Expressed in epithelial and fibroblastic cells.
  • 序列相似性Belongs to the syndecan proteoglycan family.
  • 翻译后修饰Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • 细胞定位Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amphiglycan antibody
    • MGC22217 antibody
    • OTTHUMP00000031788 antibody
    • ryudocan amphiglycan antibody
    • Ryudocan antibody
    • Ryudocan core protein antibody
    • SDC 4 antibody
    • Sdc4 antibody
    • SDC4_HUMAN antibody
    • SYND 4 antibody
    • SYND4 antibody
    • syndecan 4 antibody
    • syndecan 4 (amphiglycan, ryudocan) antibody
    • syndecan proteoglycan 4 antibody
    • Syndecan-4 antibody
    • Syndecan4 antibody
    see all

Anti-Syndecan 4 antibody 图像

  • Anti-Syndecan 4 antibody (ab24511) + Jurkat Cell lysate

    Predicted band size : 22 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • ab24511 (1µg/ml) staining Syndecan 4 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartment in the subset of intestinal glands.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab24511 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24511, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of mature rat intervertebral disc tissue, staining Syndecan 4 with ab24511.

    Tissue was fixed in 4% paraformaldehyde before incubating with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.

Anti-Syndecan 4 antibody (ab24511)参考文献

This product has been referenced in:
  • Ju R  et al. Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways. J Biol Chem 289:510-9 (2014). WB ; Mouse . Read more (PubMed: 24235146) »
  • Coulson-Thomas VJ  et al. Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and homeostasis. J Biol Chem 289:25211-26 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25053416) »

See all 11 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 10 µg
Specification MEF
Gel Running Conditions Reduced Denaturing (4%-12%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 05 2009

Thank you once again for your recent enquiry. I have contacted the originator of this antibody and can confirm it was tested on mouse liver sections in IHC-P. They have provided the testing procedure, which I have copied below. I hope this will b...

Read More

Thank you for your enquiry. The antibody has been tested with mouse liver tissue. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"