Anti-Syndecan 4抗体(ab104568)

概述

  • 产品名称Anti-Syndecan 4抗体
    参阅全部 Syndecan 4 一抗
  • 描述
    兔多克隆抗体to Syndecan 4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Dog, Human, Pig, Baboon, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.

  • 阳性对照
    • This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab104568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能Cell surface proteoglycan that bears heparan sulfate.
  • 组织特异性Expressed in epithelial and fibroblastic cells.
  • 序列相似性Belongs to the syndecan proteoglycan family.
  • 翻译后修饰Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • 细胞定位Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amphiglycan antibody
    • MGC22217 antibody
    • OTTHUMP00000031788 antibody
    • ryudocan amphiglycan antibody
    • Ryudocan antibody
    • Ryudocan core protein antibody
    • SDC 4 antibody
    • Sdc4 antibody
    • SDC4_HUMAN antibody
    • SYND 4 antibody
    • SYND4 antibody
    • syndecan 4 antibody
    • syndecan 4 (amphiglycan, ryudocan) antibody
    • syndecan proteoglycan 4 antibody
    • Syndecan-4 antibody
    • Syndecan4 antibody
    see all

Anti-Syndecan 4 antibody 图像

  • All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml

    Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Syndecan 4 antibody (ab104568)参考文献

ab104568 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (cardiac)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 40 µg
Specification cardiac
Blocking step Licor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Feb 11 2016

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