产品名称 Anti-Syndecan 4抗体 参阅全部 Syndecan 4 一抗
兔多克隆抗体to Syndecan 4
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Rat, Cow, Dog, Human, Pig, Baboon, Macaque monkey, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.
This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
功能 Cell surface proteoglycan that bears heparan sulfate.
组织特异性 Expressed in epithelial and fibroblastic cells.
序列相似性 Belongs to the syndecan proteoglycan family.
翻译后修饰 Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
细胞定位 Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
Information by UniProt
Anti-Syndecan 4 antibody 图像
Western blot - Anti-Syndecan 4 antibody (ab104568)
All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 21 kDa Observed band size : 18 kDa ( why is the actual band size different from the predicted?) Exposure time : 20 minutes
Anti-Syndecan 4 antibody (ab104568)参考文献
has not yet been referenced specifically in any publications.
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