快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节

概述

  • 产品名称
    Anti-Syndecan 2抗体
    参阅全部 Syndecan 2 一抗
  • 描述
    兔多克隆抗体to Syndecan 2

    该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »

  • 宿主
    Rabbit
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Syndecan 2.

    (Peptide available as ab90996.)

  • 阳性对照
    • This antibody gave a posiitve signal in the following whole cell lysates: Raji; HEK293; Jurkat; HeLa; HepG2; LOVO; Caco_2.

性能

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.

靶标

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Syndecan 2 antibody (ab79978) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The Syndecan 2 protein has a predicted molecular weight of 22 kDa. The first 18 amino acids of the syndecan 2 sequence act as a signal sequence, and when cleaved the protein has a molecular weight of 19 kDa (SwissProt), therefore explaining the bands presence at a lower than expected molecular weight.

文献

This product has been referenced in:
  • Coulson-Thomas VJ  et al. Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing. Invest Ophthalmol Vis Sci 56:3004-14 (2015). Read more (PubMed: 26024086) »
  • Coulson-Thomas VJ  et al. Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and homeostasis. J Biol Chem 289:25211-26 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25053416) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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