Anti-Syndecan-1抗体[B-A38] (ab34164)

概述

  • 产品名称Anti-Syndecan-1抗体[B-A38]
    参阅全部 Syndecan-1 一抗
  • 描述
    小鼠单克隆抗体[B-A38] to Syndecan-1
  • 特异性This antibody recognises a heparan sulfate rich membrane glycoprotein
  • 经测试应用适用于: ICC/IF, WB, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    U266 cell line

  • 阳性对照
    • Bone Marrow
  • 常规说明


    This antibody stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

性能

应用

Our Abpromise guarantee covers the use of ab34164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 25053416
Functional Studies Use at an assay dependent concentration. This product contains sodium azide, we recommend removal by dialysis for functional assays.

靶标

Anti-Syndecan-1 antibody [B-A38] 图像

  • ab34164 staining Syndecan in human lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). 5µm thick sections of tissue were heated at 60°C overnight, deparaffinized with xylene and rehydrated with an ethanol series.

    Sections were stained with antibodies specific to syndecan-1 (ab34164) and EC-SOD both at a 1/500 dilution. Cy3 and Alexa Fluor® 488-conjugated secondary antibodies were used.

    Green - EC-SOD, Red - syndecan, Blue - nuclear stain, Yellow - co-localization.

Anti-Syndecan-1 antibody [B-A38] (ab34164)参考文献

This product has been referenced in:
  • Miyake M  et al. Clinical implications in the shift of syndecan-1 expression from the cell membrane to the cytoplasm in bladder cancer. BMC Cancer 14:86 (2014). IHC-P ; Human . Read more (PubMed: 24524203) »
  • Garrigues HJ  et al. KSHV attachment and entry are dependent on aVß3 integrin localized to specific cell surface microdomains and do not correlate with the presence of heparan sulfate. Virology 464-465C:118-133 (2014). ICC/IF ; Human . Read more (PubMed: 25063885) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 3% BSA and 10% normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Rhesus monkey Tissue sections (colon)
Specification colon
Permeabilization Yes - 0.1% Triton-X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 0.5%
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.25% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Spleen)
Specification Spleen
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 4°C
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Ms. Janelle Bentz

Verified customer

提交于 Sep 20 2012

Thank you for contacting us.
The immunogen for this clone was a whole cell line (U266) rather than a particular peptide. Unfortunately, we currently do not provide the cell line as a separate product.
I am very sorry I couldn’t help you furth...

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I am very pleased to hear you would like to accept our offer and test ab34164 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Both citrate (low pH) and tris (high pH)
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 21 2012

Thank you for contacting us.

Here are my suggestions for the targets you mentioned:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"