概述

  • 产品名称Anti-Syndecan-1抗体
    参阅全部 Syndecan-1 一抗
  • 描述
    兔多克隆抗体to Syndecan-1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human
  • 免疫原

    Synthetic peptide (Human) surrounding amino acid 298

  • 阳性对照
    • Mouse tissue lysate

应用

Our Abpromise guarantee covers the use of ab60199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 34 kDa).

靶标

Anti-Syndecan-1 antibody 图像

  • Anti-Syndecan-1 antibody (ab60199) at 4 µg/ml + Mouse tissue lysate

    Predicted band size : 34 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    The observed band size is larger than predicted due to haparan sulfate and chondroitin sulfate modifications.
  • Anti-Syndecan-1 antibody (ab60199) at 1/2000 dilution + lysate prepared from ScN2a cells.

    Secondary
    peroxidase-conjugated secondary
    Developed using the ECL technique

    Predicted band size : 34 kDa

    Image from Taylor DR et al, PLoS Pathog. 2009 Nov;5(11):e1000666. Epub 2009 Nov 20, Fig 6.

    Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.

Anti-Syndecan-1 antibody (ab60199)参考文献

This product has been referenced in:
  • Boeddeker SJ  et al. Syndecan-1 knockdown in endometrial epithelial cells alters their apoptotic protein profile and enhances the inducibility of apoptosis. Mol Hum Reprod N/A:N/A (2014). Human . Read more (PubMed: 24478463) »
  • Chaterji S  et al. Syndecan-1 regulates vascular smooth muscle cell phenotype. PLoS One 9:e89824 (2014). Mouse . Read more (PubMed: 24587062) »

See all 5 Publications for this product

Product Wall

Thank you for sending the additional details of your protocol and the image of your blot.

Based on your results, it appears that the band between the top two markers is showing the expected expression pattern in your WT, KO, and knockdown sam...

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Thank you for your response.

Incubation with the primary antibody either at 4 degC overnight or at RT for an hour in Western blot application should have the same results. However, depending on how much time you have for the incubation and wh...

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Thank you for your email.

I believe my colleague T. just send you the reply to your first question.

Regarding your second question, unfortunately, I have to confirm that we do not know if this antibody is suitable for IF yet. This h...

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Thank you for your enquiry and your interest in our products.

This antibody has been tested for Western blot application by ECL detection system. Incubation for 1 hr @ RT or overnight @ 4 deg C should be fine. You may find some useful general...

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Thank you for contacting us.

Here are my suggestions for the targets you mentioned:

SMC alpha actin - ab5694
Calponin - ab46794
Myosin heavy chain - ab683 (heavy chain II) or ab682 (heavy chain I)
SM22 - ab14106 Read More

Thank you for your enquiry. The WB blot on our datasheet was prepared as follows: 3T3 cells grown to confluency were detached using trypsin/EDTA. These cells were spun down and washed with PBS, then resuspended in PBS at about 5-10 million cell...

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Thank you for your enquiry. I can confirm that this product is specific for Syndecan 1. Also, based on the immunogen sequence, the antibody will not likely react with Syndecan 2, 3 & 4, as the sequence homology is not significant. I hope this in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"