Anti-Synaptotagmin (phospho T202)抗体(ab51182)

概述

  • 产品名称Anti-Synaptotagmin (phospho T202)抗体
    参阅全部 Synaptotagmin 一抗
  • 描述
    兔多克隆抗体to Synaptotagmin (phospho T202)
  • 特异性ab51182 detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Synaptotagmin around the phosphorylation site of threonine 202(R-K-TP-L-N).

  • 阳性对照
    • WB: Extracts from Hela cells. IHC-P: Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab51182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.

靶标

Anti-Synaptotagmin (phospho T202) antibody 图像

  • Anti-Synaptotagmin (phospho T202) antibody (ab51182) at 1/500 dilution + extracts from Hela cells treated with Forskolin (40nM, 30min)

    Predicted band size : 48 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ab51182, at dilution of 1/50, staining Synaptotagmin in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    Left image: No peptide.
    Right image: Phosphopeptide present.

Anti-Synaptotagmin (phospho T202) antibody (ab51182)参考文献

ab51182 has not yet been referenced specifically in any publications.

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