Anti-Synaptotagmin抗体[ASV30] (ab13259)


  • 产品名称
    参阅全部 Synaptotagmin 一抗
  • 描述
    小鼠单克隆抗体[ASV30] to Synaptotagmin
  • 经测试应用
    适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Rat brain synaptic junctional protein complexes.

  • 阳性对照
    • Mouse or Rat brain tissue extract.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab13259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.


  • 功能
    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
  • 序列相似性
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • 结构域
    The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with Stonin 2.
  • 细胞定位
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HsT1192 antibody
    • MYSPC antibody
    • P65 antibody
    • SVP65 antibody
    • synaptotagmin 4 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin II antibody
    • Synaptotagmin IV antibody
    • Synaptotagmin V antibody
    • Synaptotagmin-2 antibody
    • synaptotagmin-3 antibody
    • SYT antibody
    • SYT1 antibody
    • Syt2 antibody
    • SYT2_HUMAN antibody
    • SYT3 antibody
    • SYT4 antibody
    • SYT5 antibody
    • SytII antibody
    • SytIII antibody
    • sytIV antibody
    see all


  • All lanes : Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution

    Lane 1 : Molecular weight ladder
    Lane 2 : Lysates prepared from mouse brain
    Lane 3 : Lysates prepared from rat brain
  • ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Synaptotagmin was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Synaptotagmin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13259.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
    Band: 64kDa; Synaptotagmin


This product has been referenced in:
  • Liu X  et al. Expression of P301L-hTau in mouse MEC induces hippocampus-dependent memory deficit. Sci Rep 7:3914 (2017). WB ; Mouse . Read more (PubMed: 28634382) »
  • Ceder MM  et al. The Neuronal and Peripheral Expressed Membrane-Bound UNC93A Respond to Nutrient Availability in Mice. Front Mol Neurosci 10:351 (2017). Read more (PubMed: 29163028) »

See all 7 Publications for this product


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I am sorry but this antibody has not been epitope mapped.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - other (Post nuclear rat brain lysate)
Loading amount
25 µg
Post nuclear rat brain lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C

Dr. Sriharsha Kantamneni

Verified customer

提交于 Sep 26 2007