Anti-Synaptotagmin抗体(ab51164)

概述

  • 产品名称
    Anti-Synaptotagmin抗体
    参阅全部 Synaptotagmin 一抗
  • 描述
    兔多克隆抗体to Synaptotagmin
  • 特异性
    ab51164 detects endogenous levels of total Synaptotagmin.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized non-phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309.

  • 阳性对照
    • 293 cell extract treated with PMA (125ng/ml, 30min) and human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab51164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

图片

  • Immunohistochemical analysis of human breast carcinoma tissue (paraffin section) using ab51164 (1/50 dilution); with and without peptide.
  • All lanes : Anti-Synaptotagmin antibody (ab51164) at 1/500 dilution

    Lane 1 : 293 cell extract treated with PMA (125ng/ml, 30min); without peptide
    Lane 2 : 293 cell extract treated with PMA (125ng/ml, 30min); with peptide


    Predicted band size : 48 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

文献

ab51164 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for providing the sequence and for your patience.

After aligning the sequences, I am afraid it is not very likely that any of the Anti-Synaptotagmin antibodies available will recognise the Arabidopsis protein, as the homologies are ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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