概述

  • 产品名称Anti-Synaptotagmin 4抗体
    参阅全部 Synaptotagmin 4 一抗
  • 描述
    小鼠单克隆抗体to Synaptotagmin 4
  • 经测试应用适用于: WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MAPITTSREE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR KSSKSNKTPP YKFVHVLKGV DIYPENLNSK KKFGADDKNE VKNKPAVPKN SLHLDLEKR, corresponding to amino acids 1-100 of Human Synaptotagmin 4

性能

应用

Our Abpromise guarantee covers the use of ab57473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
IHC-Fr
  • 应用说明ICC/IF: Use at an assay dependent dilution (PMID 19448629).
    IHC-Fr: Use at an assay dependent dilution (PMID 20010821).
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Synaptotagmin 4 (SYT4) belongs to the synaptotagmin family of multi-domained, integral membrane proteins of synaptic vesicles. It may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It is primarily expressed in the brain.
    • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
    • 数据库链接
    • 别名
      • HsT1192 antibody
      • KIAA1342 antibody
      • Synaptotagmin IV antibody
      • Synaptotagmin4 antibody
      • SynaptotagminIV antibody
      • SYT 4 antibody
      • Syt IV antibody
      • SYT4 antibody
      • SytIV antibody
      see all

    Anti-Synaptotagmin 4 antibody 图像



    • Predicted band size : 48 kDa
    • ICC/IF image of ab57473 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57473, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Synaptotagmin 4 antibody (ab57473)参考文献

    This product has been referenced in:
    • Johnson SL  et al. Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses. Nat Neurosci 13:45-52 (2010). IHC-Fr ; Mouse, Gerbil . Read more (PubMed: 20010821) »
    • Dean C  et al. Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release. Nat Neurosci 12:767-76 (2009). WB, ICC/IF . Read more (PubMed: 19448629) »

    See all 2 Publications for this product

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