The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Minimum dilution 1/3000. Predicted molecular weight: 100 kDa.
Staining of synaptopodin in immunoblotting is specifically inhibited with the
synaptopodin immunizing peptide (rat, amino acids 184-202).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
组织特异性Expressed in cerebral cortex.
序列相似性Belongs to the synaptopodin family.
翻译后修饰O-glycosylated. Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
细胞定位Cytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.