概述

  • 产品名称
    Anti-Synaptopodin抗体
    参阅全部 Synaptopodin 一抗
  • 描述
    兔多克隆抗体to Synaptopodin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Synthetic peptide:

    GQRSPKLSQEALQTGHPSS

    conjugated to KLH, corresponding to internal sequence amino acids 184-202 of Rat Synaptopodin

  • 阳性对照
    • Cytosolic S1 fraction of rat brain.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    Whole antiserum was fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Minimum dilution 1/3000. Predicted molecular weight: 100 kDa.
    Staining of synaptopodin in immunoblotting is specifically inhibited with the
    synaptopodin immunizing peptide (rat, amino acids 184-202).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
    • 组织特异性
      Expressed in cerebral cortex.
    • 序列相似性
      Belongs to the synaptopodin family.
    • 翻译后修饰
      O-glycosylated.
      Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
    • 细胞定位
      Cytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA1029 antibody
      • Synaptopodin antibody
      • SYNPO antibody
      • SYNPO_HUMAN antibody

    实验方案

    Anti-Synaptopodin antibody (ab50859)参考文献

    This product has been referenced in:
    • Moriguchi T  et al. Gata3 Hypomorphic Mutant Mice Rescued with a Yeast Artificial Chromosome Transgene Suffer a Glomerular Mesangial Cell Defect. Mol Cell Biol 36:2272-81 (2016). IHC ; Mouse . Read more (PubMed: 27296697) »
    • Reissner KJ  et al. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. J Neurosci 31:5648-58 (2011). WB ; Rat . Read more (PubMed: 21490206) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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