RabMAb

Anti-Synaptophysin抗体[YE269] (ab32127)

概述

性能

应用

Our Abpromise guarantee covers the use of ab32127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).
ICC/IF 1/50.

For unpurified, use at 1/250.

IHC-Fr Use at an assay dependent concentration.
IHC-P 1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 组织特异性Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 疾病相关Mental retardation, X-linked, SYP-related
  • 序列相似性Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • 结构域The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody [YE269] 图像

  • All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Secondary
    HRP goat anti-rabbit at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

  • Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified) + Human fetal brain at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-Synaptophysin antibody [YE269] (ab32127) at 1/10000 dilution (unpurified) + PC12 cell lysate

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 21 kDa,22 kDa. We are unsure as to the identity of these extra bands.
  • Unpurified ab32127 showing positive staining in Medulloblastoma tissue.

  • Unpurified ab32127 showing positive staining in Lung neuroendocrine tumor tissue.

Anti-Synaptophysin antibody [YE269] (ab32127)参考文献

This product has been referenced in:
  • Atzpodien EA  et al. Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs. Toxicol Pathol 44:398-413 (2016). Read more (PubMed: 26680760) »
  • Shin CH  et al. The BRAF kinase domain promotes the development of gliomas in vivo. Genes Cancer 6:9-18 (2015). IHC-P ; Mouse . Read more (PubMed: 25821557) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
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提交于 Dec 05 2016

Application IHC - Wholemount
Sample Mouse Tissue (Diaphragm)
Specification Diaphragm
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提交于 Oct 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (neurons from iPS cells)
Gel Running Conditions Non-reduced Denaturing (12%)
Loading amount 20 µg
Specification neurons from iPS cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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���交于 Jan 15 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization Yes - 0.1% triton x-100
Fixative Paraformaldehyde
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提交于 Oct 06 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Donkey Tissue sections (brain slice)
Specification brain slice
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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提交于 Sep 08 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Cat Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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提交于 Sep 08 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Chicken Tissue sections (Brain stem)
Specification Brain stem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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提交于 Sep 08 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Mouse Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.5% triton
Fixative Paraformaldehyde
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提交于 Aug 18 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Rat Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.5% triton
Fixative Paraformaldehyde
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提交于 Aug 18 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (Human Cell (human iPS cell derived neurons))
Specification Human Cell (human iPS cell derived neurons)
Permeabilization Yes - see blocking buffer
Fixative Paraformaldehyde
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提交于 Jul 10 2014

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