概述

  • 产品名称Anti-Synaptophysin抗体
    参阅全部 Synaptophysin 一抗
  • 描述
    兔多克隆抗体to Synaptophysin
  • 特异性ab53166 detects endogenous levels of total Synaptophysin protein.
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide derived from human Synaptophysin.

  • 阳性对照
    • IHC-P: Human brain tissue. WB: Extracts from LOVO cells. ICC/IF formaldehyde fixed SKNSH cells.

性能

应用

Our Abpromise guarantee covers the use of ab53166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 组织特异性Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 疾病相关Mental retardation, X-linked, SYP-related
  • 序列相似性Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • 结构域The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody 图像

  • All lanes : Anti-Synaptophysin antibody (ab53166) at 1/500 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide


    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab53166 at 1/50 dilution staining Synaptophysin in Human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
  • ICC/IF image of ab53166 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53166 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Synaptophysin antibody (ab53166)参考文献

This product has been referenced in:
  • Arnold AS  et al. Morphological and functional remodelling of the neuromuscular junction by skeletal muscle PGC-1a. Nat Commun 5:3569 (2014). WB . Read more (PubMed: 24686533) »
  • Lo AC  et al. Dose-dependent improvements in learning and memory deficits in APPPS1-21 transgenic mice treated with the orally active Aß toxicity inhibitor SEN1500. Neuropharmacology 75C:458-466 (2013). Read more (PubMed: 24035915) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"