Anti-Synaptophysin抗体(ab32594)

概述

  • 产品名称Anti-Synaptophysin抗体
    参阅全部 Synaptophysin 一抗
  • 描述
    兔多克隆抗体to Synaptophysin
  • 经测试应用适用于: WB, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Zebrafish, Apteronotus leptorhynchus
    预测可用于: Human
  • 免疫原

    Synthetic peptide:

    GPGGYGPQDSYGPQGGYQPD

    , corresponding to amino acids 253-272 of Rat Synaptophysin

  • 阳性对照
    • Rat, mouse and bovine brain tissue and sheep retinas.

性能

应用

Our Abpromise guarantee covers the use of ab32594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 组织特异性Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 疾病相关Mental retardation, X-linked, SYP-related
  • 序列相似性Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • 结构域The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody 图像

  • ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg

    Secondary
    AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 33,35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 7 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain

    Predicted band size : 34 kDa
    Observed band size : 33.8 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Apteronotus leptorhynchus brain tissue, staining Synaptophysin with ab32594.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% sheep serum, 1% BSA and 1% teleostean gelatine in TBS for 1 hour at 24°. Samples were incubated with primary antibody (1/100 in blocking solution) for 18 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

Anti-Synaptophysin antibody (ab32594)参考文献

This product has been referenced in:
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . Read more (PubMed: 27662313) »
  • Ribeiro LF  et al. Ghrelin triggers the synaptic incorporation of AMPA receptors in the hippocampus. Proc Natl Acad Sci U S A 111:E149-58 (2014). Read more (PubMed: 24367106) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (hippocampal nueron)
Specification hippocampal nueron
Permeabilization Yes - triton x-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 13 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Mouse Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Permeabilization Yes - 1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (Brain)
Loading amount 10 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ayça Arslan Ergül

Verified customer

提交于 Dec 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Nov 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (brain)
Loading amount 21 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10% resolving SDS-PAGE gel)
Blocking step Milk as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mrs. Fusun Doldur Balli

Verified customer

提交于 Apr 12 2012

Thank you for contacting me and letting me know about this situation.

I have entered your Abreview codes into the corresponding reviews, so these are now active and can be redeemed for free primary antibodies.

I am sorry to see that...

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Thank you for your reply.

You can submit an Abreview by logging into your Abcam account and clicking on "Abreviews" on the left side of the screen underneath "My Account". Then, click on "Write an Abreview" and s...

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Thank you for contacting us and for your patience while I have reviewed the situation. I am sorry to see that the resultshave not been optimal with these antibodies, but I am hopeful that we can improve the blots.

1)With the synaptophysin ant...

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Thank you for your reply. I am still working on the alignments for some of your targets, but I have some antibodies that might interest you. We do not have any antibodies to the targets you listed which have been validated for use with zebrafis...

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