Anti-Synaptojanin抗体- Synaptic Marker (ab19904)

概述

  • 产品名称Anti-Synaptojanin抗体- Synaptic Marker
    参阅全部 Synaptojanin 一抗
  • 描述
    兔多克隆抗体to Synaptojanin - Synaptic Marker
  • 经测试应用适用于: WB, ICC/IF, IHC-FoFr, IPmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1250 - 1350 of Rat Synaptojanin.

    (Peptide available as ab20433.)

  • 阳性对照
    • rat brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab19904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr 1/300.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.
  • 组织特异性Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.
  • 序列相似性Belongs to the synaptojanin family.
    In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAC domain.
  • 结构域Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
    The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4 antibody
    • 5-trisphosphate 5-phosphatase 1 antibody
    • Inositol 5' phosphatase (synaptojanin 1) antibody
    • Inositol 5' phosphatase antibody
    • INPP 5G antibody
    • INPP5G antibody
    • KIAA0910 antibody
    • PARK20 antibody
    • Polyphosphoinositide phosphatase antibody
    • Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1 antibody
    • Synaptic inositol 145 trisphosphate 5 phosphatase 1 antibody
    • Synaptic inositol-1 antibody
    • Synaptojanin 1 antibody
    • Synaptojanin 1 polyphosphoinositide phosphatase antibody
    • Synaptojanin-1 antibody
    • Synaptojanin1 antibody
    • Synj 1 antibody
    • Synj1 antibody
    • SYNJ1_HUMAN antibody
    see all

Anti-Synaptojanin antibody - Synaptic Marker 图像

  • All lanes : Anti-Synaptojanin antibody - Synaptic Marker (ab19904) at 1 µg/ml

    Lane 1 : Rat Brain lysate
    Lane 2 : Rat Brain lysate with Rat Synaptojanin peptide (ab20433) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Rabbit polyclonal to Goat IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 150 kDa
    Observed band size : 150 kDa
    ab19904 detected the expected 150kDa band in non-boiled (rat brain lysate) sample only and the detection of this band was blocked by addition of immunising peptide ab20433
  • ICC/IF image of ab19904 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab19904, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemistical detection of Synaptojanin using ab19904 on mouse brain PFA perfusion-fixed sections. Primary antibody ab19904 diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488® (1/1000). Image: immunostaining obtained in the mouse hippocampus on perfusion-fixed free floating sections using ab19904 at 1/300 using direct fluorescence. The staining is so strong that it is hard to determine the cellular localisation, but it seems to be cytoplasmic.

    See Abreview

  • Immunohistochemical analysis of PFA-perfusion fixed mouse brain tissue, staining Synaptojanin with ab19904.

    Tissue was post-fixed in 4% paraformaldehyde overnight at 4°C. Sections were incubated in a blocking buffer of 0.3% Triton-X-100, 1% BSA and 10% NGS in PBS for 2 hours at room temperature, before incubating with primary antibody (1/100) for 2 days at 4°C. An AlexaFluor®-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
  • Synaptojanin was immunoprecipitated using 0.5mg Rat brain tissue, 5µg of Rabbit polyclonal to Synaptojanin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19904.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 150kDa; Synaptojanin

Anti-Synaptojanin antibody - Synaptic Marker (ab19904)参考文献

This product has been referenced in:
  • Wagnon JL  et al. CELF4 regulates translation and local abundance of a vast set of mRNAs, including genes associated with regulation of synaptic function. PLoS Genet 8:e1003067 (2012). IHC-FoFr, WB ; Mouse . Read more (PubMed: 23209433) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 02 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Hippocampal neuron culture)
Specification Hippocampal neuron culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Jun 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"