Anti-Synaptogyrin 4抗体(ab27480)

概述

  • 产品名称
    Anti-Synaptogyrin 4抗体
    参阅全部 Synaptogyrin 4 一抗
  • 描述
    山羊多克隆抗体to Synaptogyrin 4
  • 宿主
    Goat
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    HIPKSLQELANSC

    , corresponding to amino acids 2-14 of Human Synaptogyrin 4

  • 阳性对照
    • Human brain lysate for Western blot.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: 1/128000.
    WB: Use at a concentration of 0.01 - 01 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. Can be blocked with Synaptogyrin 4 peptide (2-14) (ab45658).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • Anti-Synaptogyrin 4 antibody (ab27480) at 0.01 µg/ml + Human brain lysate (35µg protein in RIPA buffer).

      Predicted band size: 26 kDa
      Observed band size: 23 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.



      Primary incubation was 1 hour. Detected by chemiluminescence. A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide.

    实验方案

    文献

    ab27480 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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