概述

  • 产品名称Anti-Synapsin II抗体
    参阅全部 Synapsin II 一抗
  • 描述
    兔多克隆抗体to Synapsin II
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide:

    ASPGPERRPPPAQA

    conjugated to KLH, corresponding to amino acids 52-65 of Rat Synapsin II. This sequence is identical in synapsin IIa and IIb.

  • 阳性对照
    • Rat brain tissue extract.

性能

应用

Our Abpromise guarantee covers the use of ab13258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 74, 54 kDa (predicted molecular weight: 57.5 kDa).
IP 1/100.
ICC/IF Use at an assay dependent dilution. PubMed: 19714568

靶标

  • 相关性Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.
  • 细胞定位Cell junction, synapse.
  • 数据库链接
  • 别名
    • SYN 2 antibody
    • SYN II antibody
    • SYN IIa antibody
    • SYN IIb antibody
    • SYN2 antibody
    • Synapsin 2 antibody
    • Synapsin II isoform IIa antibody
    • Synapsin II isoform IIb antibody
    • Synapsin2 antibody
    • SynapsinII antibody
    • SYNII antibody
    • SYNIIa antibody
    • SYNIIb antibody
    see all

Anti-Synapsin II antibody 图像

  • Anti-Synapsin II antibody (ab13258) at 1/2000 dilution + Rat whole brain tissue lysate at 15 µg

    Secondary
    HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57.5 kDa
    Observed band size : 60,78 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 25°C.

    Incubated with the primary antibody diluted in TBS + 0.1% Tween for 18 hours at 4°C.

    See Abreview



  • Predicted band size : 57.5 kDa

    Image from Ramos C et al., J Biol Chem. 2012 Jun 8;287(24):20176-86. doi: 10.1074/jbc.M112.347468. Epub 2012 Apr 23. Fig 5.; June 8, 2012 The Journal of Biological Chemistry, 287, 20176-20186.

    Western blot analysis of rat cerebellum tissue, staining Synapsin II with ab13258.

    Samples were immunoprecipitated with antibodies against CB or mGluR4 and co-immunoprecipitated Synapsin II was detected using ab13258. Synapsin I was detected using ab8 - Rabbit polyclonal to Synapsin I.

Anti-Synapsin II antibody (ab13258)参考文献

This product has been referenced in:
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF . Read more (PubMed: 27592818) »
  • Ramos C  et al. Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum. J Biol Chem 287:20176-86 (2012). WB ; Rat . Read more (PubMed: 22528491) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (whole brain)
Gel Running Conditions Reduced Denaturing (7.5% Tris gel)
Loading amount 15 µg
Specification whole brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 01 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"