RabMAb

Anti-Synapsin I (phospho S553)抗体[E377] (ab32532)

概述

  • 产品名称Anti-Synapsin I (phospho S553)抗体[E377]
    参阅全部 Synapsin I 一抗
  • 描述
    兔单克隆抗体[E377] to Synapsin I (phospho S553)
  • 特异性This antibody only detects Synapsin phosphorylated on Serine 553
  • 经测试应用适用于: ICC/IF, WB, Dot blotmore details
    不适用于: Flow Cyt,IHC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Ser553 of Synapsin (Human)

  • 阳性对照
    • Mouse brain lysates, PC-12 cells
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Dot blot 1/500.
  • 应用说明Is unsuitable for Flow Cyt,IHC or IP.
  • 靶标

    • 功能Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
    • 疾病相关Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
    • 序列相似性Belongs to the synapsin family.
    • 翻译后修饰Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cell junction > synapse. Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Brain protein 4.1 antibody
      • SYN 1 antibody
      • SYN 1a antibody
      • SYN 1b antibody
      • SYN I antibody
      • SYN1 antibody
      • SYN1_HUMAN antibody
      • SYN1a antibody
      • SYN1b antibody
      • Synapsin 1 antibody
      • Synapsin I antibody
      • Synapsin-1 antibody
      • Synapsin1 antibody
      • SynapsinI antibody
      • SYNI antibody
      see all

    Anti-Synapsin I (phospho S553) antibody [E377] 图像

    • All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

      Lane 1 : Untreated rat brain whole cell lysates
      Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second
    • All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

      Lane 1 : Untreated mouse brain whole cell lysate
      Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution

      Lane 1 : Untreated mouse brain whole cell lysate
      Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer: 2% BSA/TBST

      Dilution buffer: 2% BSA/TBST

    • Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500
    • Immunofluorescent staining of PC-12 cells using ab32532 at a dilution of 1/50 -1/100

    Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)参考文献

    This product has been referenced in:
    • Sriram K  et al. Dopaminergic neurotoxicity following pulmonary exposure to manganese-containing welding fumes. Arch Toxicol 84:521-40 (2010). WB ; Rat . Read more (PubMed: 20224926) »

    See 1 Publication for this product

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