重组Anti-Synapsin I (phospho S553)抗体[E377] (ab32532)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E377] to Synapsin I (phospho S553)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Synapsin I (phospho S553)抗体[E377]
参阅全部 Synapsin I 一抗 -
描述
兔单克隆抗体[E377] to Synapsin I (phospho S553) -
宿主
Rabbit -
特异性
This antibody only detects Synapsin phosphorylated on Serine 553 -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt,ICC/IF,IHC or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Untreated Human fetal brain, Mouse brain lysates, and Human fetal brain treated with alkaline phosphatase lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E377 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32532于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
|
Dot blot |
1/500.
|
说明 |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
Dot blot
1/500. |
靶标
-
功能
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
疾病相关
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
序列相似性
Belongs to the synapsin family. -
翻译后修饰
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cell junction > synapse. Golgi apparatus. - Information by UniProt
-
数据库链接
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
别名
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
图片
-
All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution (Purified)
Lane 1 : Untreated Human fetal brain lysates
Lane 2 : Human fetal brain lysates treated with alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution
Lane 1 : Untreated rat brain whole cell lysates
Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
-
All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution
Lane 1 : Untreated mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution
Lane 1 : Untreated mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
-
Dot Blot analysis on human immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (13)
ab32532 被引用在 13 文献中.
- Fu Y et al. Loss of neurodevelopmental-associated miR-592 impairs neurogenesis and causes social interaction deficits. Cell Death Dis 13:292 (2022). PubMed: 35365601
- von Ziegler LM et al. Multiomic profiling of the acute stress response in the mouse hippocampus. Nat Commun 13:1824 (2022). PubMed: 35383160
- Wang Y et al. Roles of Rufy3 in experimental subarachnoid hemorrhage-induced early brain injury via accelerating neuronal axon repair and synaptic plasticity. Mol Brain 15:35 (2022). PubMed: 35461284
- Montenegro-Venegas C et al. Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP. EMBO Rep 23:e53659 (2022). PubMed: 35766170
- Xiang Y et al. Oxidative stress-induced premature senescence and aggravated denervated skeletal muscular atrophy by regulating progerin-p53 interaction. Skelet Muscle 12:19 (2022). PubMed: 35906707