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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat Synapsin I aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab64581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa).
Abcam recommends using 3-5% milk as the blocking agent.
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Blocked with 5% milk for 1 hour at 25°C.
Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.
ab64581 staining cultured rat hippocampal neurons by ICC/IF. The cultured neurons were fixed with methanol, permeabilized with 0.3% Triton X in 0.1% PBS and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured neurons were then stained with ab64581 at 1/200 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with Hoechst and can be observed in blue.
ab64581 staining Synapsin I in rat brain (cerebellum) tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.01% Triton X-100, blocked with 10% serum for 2 hours at 25°C; antigen retrieval was by heat mediation in a citrate buffer. The sample was incubated with the primary antibody (1/300) at 25°C for 12 hours. An undiluted Alexa Fluor® 555-conjugated goat anti-rabbit polyclonal was used as the secondary antibody.
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