概述

  • 产品名称Anti-Synapsin I抗体
    参阅全部 Synapsin I 一抗
  • 描述
    兔多克隆抗体to Synapsin I
  • 经测试应用适用于: WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Synapsin I. Corresponds to residues surrounding amino acid 12 of Synapsin I.

  • 阳性对照
    • WB: Human, mouse and rat brain lysates.

性能

应用

Our Abpromise guarantee covers the use of ab18814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 74 kDa).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22579680

靶标

  • 功能Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • 疾病相关Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • 序列相似性Belongs to the synapsin family.
  • 翻译后修饰Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

Anti-Synapsin I antibody 图像

  • All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

    Lane 1 : Human brain whole cell lysate - untreated
    Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 74 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking and dilution buffer: 5% NFDM/TBST.

    Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

  • All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

    Lane 1 : Mouse brain whole cell lysate - untreated
    Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 74 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking and dilution buffer: 5% NFDM/TBST.

    Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

  • All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

    Lane 1 : Rat brain whole cell lysate - untreated
    Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 74 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 seconds

    Blocking and dilution buffer: 5% NFDM/TBST.

    Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

  • Anti-Synapsin I antibody (ab18814) at 1 µg/ml + Mouse brain tissue lysate.

    Predicted band size : 74 kDa
    Observed band size : ~77 kDa (why is the actual band size different from the predicted?)

    Secondary antibody - goat anti-rabbit HRP (ab6721)

Anti-Synapsin I antibody (ab18814)参考文献

This product has been referenced in:
  • Gulino R & Gulisano M Involvement of brain-derived neurotrophic factor and sonic hedgehog in the spinal cord plasticity after neurotoxic partial removal of lumbar motoneurons. Neurosci Res 73:238-47 (2012). WB, IHC-FoFr ; Mouse . Read more (PubMed: 22579680) »
  • Lagarrigue M  et al. New Analysis Workflow for MALDI Imaging Mass Spectrometry: Application to the Discovery and Identification of Potential Markers of Childhood Absence Epilepsy. J Proteome Res 11:5453-63 (2012). Read more (PubMed: 22994238) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 2 µg
Gel Running Conditions Non-reduced Denaturing
Sample Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification hippocampus tissue lysate
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Jun 18 2014

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Sunetra Jitkar

Verified customer

提交于 Feb 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole brain)
Loading amount 20 µg
Specification Whole brain
Gel Running Conditions Reduced Denaturing (gel 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2012

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (hippocampus)
Loading amount 5 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"