概述

  • 产品名称
    Anti-Symplekin抗体
    参阅全部 Symplekin 一抗
  • 描述
    兔多克隆抗体to Symplekin
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 350 and 400 of Human Symplekin (NP_004810.2).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab80274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 165 kDa (predicted molecular weight: 141 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性
    Symplekin (SYMPK) can associate with tight junctions, functions in the regulation of polyadenylation and promotes gene expression. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation.
  • 细胞定位
    Cytoplasm, cytoskeleton. Cell junction, tight junction; Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells.
  • 数据库链接
  • 别名
    • FLJ27092 antibody
    • SPK antibody
    • SYM antibody
    • SYMPK antibody

图片

  • All lanes : Anti-Symplekin antibody (ab80274) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 141 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)
  • Detection of Symplekin in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab80274 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

文献

This product has been referenced in:
  • Soboleva TA  et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . Read more (PubMed: 28234895) »

See 1 Publication for this product

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