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Recombinant fragment, corresponding to amino acids 5-360 of Human Syk.
Our Abpromise guarantee covers the use of ab3993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.|
Immunofluorescence staining of Syk in human HeLa (human epithelial cell line from cervix adenocarcinoma) cell line using ab3993 (green) at 5 µg/ml. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunofluorescence staining of Syk in human primary fibroblasts using ab3993 (green) at 5 μg/ml. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Image from Zou W et al, J Biol Chem. 2009 Jul 10;284(28):18833-9. Epub 2009 May 6, Fig 1. DOI 10.1074/jbc.M109.012385Transduced Syk-/- BMMs, cultured in 50 ng/ml M-CSF for 3 days, were serum- and cytokine-starved overnight. The cells were then exposed to either 100 ng/ml M-CSF with time. Signaling molecules were identified by immunoblotting. Actin serves as a loading control.