Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region of Human SYDE1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Anti-SYDE1 antibody 图像
Western blot - Anti-SYDE1 antibody (ab80402)
Predicted band size : 80 kDa Western blot analysis of Human fetal liver tissue lysate labeling SYDE1 with ab80402 at 1.0µg/ml.
Western blot - SYDE1 antibody (ab80402)
Anti-SYDE1 antibody (ab80402) at 1 µg/ml + Hela cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 80 kDa Observed band size : 80 kDa Gel concentration: 12%
Anti-SYDE1 antibody (ab80402)参考文献
This product has been referenced in:
et al. In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes. J Biol Chem 288:19816-29 (2013).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"