The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus (S-OIV) was identified in specimens obtained from patients in Mexico and the United States. One of the less studied proteins encoded by, but not incorporated in, the influenza virus is the Swine H1N1 Nonstructural Protein 1. It counters cellular antiviral activities and acts as a virulence factor. It can bind to double-stranded RNA and sequester it from 2’-5’ OAS, preventing the activation of the RNAse L, which normally acts to degrade RNA and prevent virus replication. It also binds to and inhibits the anti-viral protein kinase PKR.
S-OIV NS1 antibody
Swine origin Influenza A (H1N1) Nonstructural Protein 1 antibody
ELISA - Swine H1N1 Nonstructural Protein 1 antibody (ab91647)
ab91647, at a concentration of 1 µg/ml, detects swine-origin H1N1 Nonstructural Protein 1 peptide. It does not crossreact with peptide corresponding to the seasonal H1N1 Nonstructural Protein 1. The blocking and corresponding peptides were used at 60, 10, 2 and 0 ng/ml.
has not yet been referenced specifically in any publications.