SWAP70 overexpression 293T裂解液(全细胞) (ab94234)

概述

  • 产品名称SWAP70 overexpression 293T裂解液(全细胞)
  • 描述
    SWAP70 overexpression裂解液
  • 常规说明ab94234 is a 293T cell transfected lysate in which Human SWAP70 has been transiently over-expressed using a pCMV-SWAP70 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. Tissue specificity: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760). Similarity: Contains 1 PH domain. Domain: The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding. PTM: Tyrosine-phosphorylated.

应用

Our Abpromise guarantee covers the use of ab94234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

SWAP70 overexpression 293T lysate (whole cell) 图像

  • ab94234 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SWAP70 antibody (ab89532) at 1/500 dilution

    Lane 1 : SWAP70 overexpression 293T lysate (whole cell) (ab94234)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SWAP70 overexpression 293T lysate (whole cell) (ab94234)参考文献

ab94234 has not yet been referenced specifically in any publications.

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