概述
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产品名称Anti-SWAP70抗体
参阅全部 SWAP70 一抗 -
描述小鼠多克隆抗体to SWAP70
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
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免疫原
Full length Human SWAP70 (NP_055870.2, amino acids 1-585).
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阳性对照
- Human spleen tissue lysate, HepG2 cell lysate, SWAP70 transfected 293T cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab89605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. |
靶标
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功能Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
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组织特异性Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
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序列相似性Contains 1 PH domain.
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结构域The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
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翻译后修饰Tyrosine-phosphorylated.
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细胞定位Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
- Information by UniProt
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数据库链接
- Entrez Gene: 23075 Human
- Omim: 604762 Human
- SwissProt: Q9UH65 Human
- Unigene: 153026 Human
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别名
- KIAA0640 antibody
- 70kDa antibody
- AV235546 antibody
see all
Anti-SWAP70 antibody 图像
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Western blot - SWAP70 antibody (ab89605)Anti-SWAP70 antibody (ab89605) at 1 µg/ml + Human spleen tissue lysate at 50 µg
Predicted band size : 69 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?) -
Western blot - SWAP70 antibody (ab89605)Anti-SWAP70 antibody (ab89605) at 1 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size : 69 kDa
Observed band size : 72 kDa (why is the actual band size different from the predicted?) -
Western blot - SWAP70 antibody (ab89605)All lanes : Anti-SWAP70 antibody (ab89605) at 1 µg/ml
Lane 1 : SWAP70 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 69 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
Anti-SWAP70 antibody (ab89605)参考文献
ab89605 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"