重组Anti-SVH抗体[EPR13763] - C-terminal (ab188573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13763] to SVH - C-terminal
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SVH抗体[EPR13763] - C-terminal
参阅全部 SVH 一抗 -
描述
兔单克隆抗体[EPR13763] to SVH - C-terminal -
宿主
Rabbit -
特异性
Based on the sequence analysis, this antibody recognizes 5 isoforms with the predicted MWs of 38KDa, 34KDa, 31KDa, 27KDa and 31KDa, respectively.
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经测试应用
适用于: ICC/IF, IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- SKBR-3, A431, HepG2, U87-MG, C6, Raw264.7, PC-12, NIH3T3 lysates; A431 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13763 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50.
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IP |
1/50.
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|
WB |
1/1000. Detects a band of approximately 27, 34 kDa (predicted molecular weight: 38, 34, 31, 27, 31 kDa).
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说明 |
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ICC/IF
1/50. |
IP
1/50. |
WB
1/1000. Detects a band of approximately 27, 34 kDa (predicted molecular weight: 38, 34, 31, 27, 31 kDa). |
靶标
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功能
May play a role in cell survival and cell growth. May suppress the transcriptional activity of p53/TP53. -
组织特异性
Expressed in all tissues tested with higher expression in placenta, liver, kidney, heart and brain. -
序列相似性
Contains 1 ARM repeat. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 83787 Human
- Entrez Gene: 67211 Mouse
- Entrez Gene: 296758 Rat
- Omim: 611864 Human
- SwissProt: Q8N2F6 Human
- SwissProt: Q9D0L7 Mouse
- SwissProt: B1WBW4 Rat
- Unigene: 287412 Human
see all -
别名
- ARM10_HUMAN antibody
- Armadillo repeat containing 10 antibody
- Armadillo repeat containing protein 10 antibody
see all
图片
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All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilution
Lane 1 : SKBR-3 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38, 34, 31, 27, 31 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed A431cells labeling SVH with ab188573 at 1/50 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.
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All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilution
Lane 1 : C6 lysate
Lane 2 : RAW264.7 lysate
Lane 3 : PC-12 lysate
Lane 4 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38, 34, 31, 27, 31 kDa -
Western blot analysis of SVH in immunoprecipitation pellets from U87-MG lysate. ab188573 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.
All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilution
Lane 1 : U87-MG
Lane 2 : PBS instead of U87-MG lysates
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Additional bands at: 27, 34 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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