SV40 VP2 + VP3总蛋白裂解液- Positive Control (ab170088)

概述

  • 产品名称
    SV40 VP2 + VP3总蛋白裂解液- Positive Control
  • 常规说明
    Wild type SV40 was propagated on CV1 cells and harvested by the di-detergent protocol (Arad et al., (2005) Human Gene Therapy 16: 361-371).
  • 经测试应用
    适用于: WBmore details

性能

  • 形式
    Liquid
  • 存放说明
    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 研究领域
  • 背景
    There are three simian virus 40 (SV40) capsid proteins - VP1, VP2 and VP3. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.

应用

Our Abpromise guarantee covers the use of ab170088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Add 1/10 volume of beta-mercaptoethanol reagent to the loading buffer to dissociate Vp1 oligomers.

图片

  • All lanes : Anti-SV40 VP2 + VP3 antibody (ab53983)

    Lane 1 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 10 µl
    Lane 2 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 3.3 µl
    Lane 3 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 0.7 µl
    Lane 4 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 0.3 µl
  • All lanes : Anti-SV40 VP1 antibody (ab53977)

    Lane 1 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 10 µl
    Lane 2 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 3.3 µl
    Lane 3 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 0.7 µl
    Lane 4 : SV40 VP2 + VP3 Total Protein Lysate - Positive Control (ab170088) at 0.3 µl

文献

ab170088 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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