概述

  • 产品名称Anti-SV2C抗体
    参阅全部 SV2C 一抗
  • 描述
    兔多克隆抗体to SV2C
  • 经测试应用适用于: ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat SV2C.

    (Peptide available as ab33891.)

  • 阳性对照
    • ab33892 gave a positive signal in Liver (Rat) Tissue Lysate. This antibody also gave a weak signal in Brain (Rat) Tissue Lysate (data not shown).

性能

应用

Our Abpromise guarantee covers the use of ab33892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-FoFr
WB
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern; the highest expression levels being observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb.
    • 细胞定位Integral Membrane Protein
    • 数据库链接
    • 别名
      • Synaptic vesicle protein 2c antibody

    Anti-SV2C antibody 图像

    • Anti-SV2C antibody (ab33892) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 82 kDa
      Observed band size : 82 kDa
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab33892 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33892, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • ab33892 staining SV2C in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. The picture was taken at level of hippocampus.

      See Abreview

    Anti-SV2C antibody (ab33892)参考文献

    This product has been referenced in:
    • Couesnon A  et al. Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine. PLoS Pathog 8:e1002583 (2012). WB ; Mouse . Read more (PubMed: 22438808) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Jan 29 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"