The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 83 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
Belongs to the major facilitator superfamily.
Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1. N-glycosylated.
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas.
Primary incubation was 1 hour. Detected by chemiluminescence.
An additonal band of unknown identity was consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide.