Anti-SUV420h1抗体- ChIP Grade (ab118659)

概述

  • 产品名称Anti-SUV420h1抗体- ChIP Grade
    参阅全部 SUV420h1 一抗
  • 描述
    兔多克隆抗体to SUV420h1 - ChIP Grade
  • 经测试应用适用于: ChIP, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Guinea pig, Cow, Cat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 271-320 (FINHDCRPNC KFVSTGRDTA CVKALRDIEP GEEISCYYGD GFFGENNEFC) of Mouse SUV420h1 (NP_001161356).

  • 阳性对照
    • Mouse Thymus lysate.

性能

应用

Our Abpromise guarantee covers the use of ab118659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.
  • 序列相似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
    Contains 1 SET domain.
  • 细胞定位Nucleus. Chromosome. Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CGI 85 antibody
    • CGI85 antibody
    • Histone-lysine N-methyltransferase KMT5B antibody
    • Kmt5b antibody
    • KMT5B_HUMAN antibody
    • Lysine N-methyltransferase 5B antibody
    • Lysine-specific methyltransferase 5B antibody
    • MGC118906 antibody
    • MGC118909 antibody
    • MGC21161 antibody
    • MGC703 antibody
    • Su(var)4-20 homolog 1 antibody
    • Suppressor of variegation 4 20 homolog 1 antibody
    • Suppressor of variegation 4-20 homolog 1 antibody
    • Suv4-20h1 antibody
    see all

Anti-SUV420h1 antibody - ChIP Grade 图像

  • ChIP analysis using ab118659 (0.5µg/mL) binding SUV420h1 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.

  • Anti-SUV420h1 antibody - ChIP Grade (ab118659) at 1 µg/ml + Mouse Thymus lysate at 10 µg

    Predicted band size : 38 kDa
    Gel concentration: 12%
  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Anti-SUV420h1 antibody - ChIP Grade (ab118659)参考文献

ab118659 has not yet been referenced specifically in any publications.

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