概述

  • 产品名称Anti-Survivin DEx3抗体
  • 描述
    兔多克隆抗体to Survivin DEx3
  • 特异性This antibody has been tested in Western blotting against equal loadings of purified wild type survivin, 2B and DEx3. When used at 1/250, it detects a single clean (but weak) band of the appropriate size for DEx3, but no band is detected with wild type survivin or 2B. We have not succeeded in detecting endogenous Survivin DEx3 using this antibody (Survivin DEx3 is expressed at very low levels). This antibody has been used successfully however, to detect overexpressed Survivin DEx3 in whole cell extracts.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide of Human Survivin DEx3, derived from amino acids 50-150.

    (Peptide available as ab13763.)

  • 阳性对照
    • This antibody gave a positive result in HeLa cell line in ICC/IF
  • 常规说明This antibody should be aliquoted to reduce freeze-thaw cycles as it loses performance quickly after multiple freeze-thaws.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性Survivin is expressed in G2/M phase in a cycle regulated manner. At the beginning of mitosis, survivin associates with mitotic spindle microtubules, this is essential for survivin's anti-apoptosis function. Survivin may counteract a default induction of apoptosis in G2 / M phase. Survivin over-expression in cancer may overcome this apoptotic checkpoint. Survivin DEx3 (lacking exon 3) and survivin-2B (retaining a part of intron 2 as a cryptic exon) are isoforms of survivin that behave differently to survivin. Skipping of exon 3 in survivin-Ex3 results in a modification of the BIR domain, followed by an additional frame shift in exon 4. In consequence, the open reading frame of Survivin DEx3 ends in the 3' untranslated region of survivin at position 581 and generates a novel 63 amino acids-long COOH-terminal tail in survivin-Ex3 coded by 87 nucleotides of exon 4 and 103 nucleotides of the original 3' untranslated region. See Mahotka et al. 1999 for more details.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • API4 antibody
    • Apoptosis Inhibitor 4 antibody
    • BIRC5 antibody
    • EPR1 antibody
    • IAP4 antibody
    • TIAP antibody
    see all

Anti-Survivin DEx3 antibody 图像

  • ICC/IF image of ab3731 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Survivin DEx3 antibody (ab3731)参考文献

This product has been referenced in:
  • Doucette T  et al. Survivin transcript variant 2 drives angiogenesis and malignant progression in proneural gliomas. Neuro Oncol N/A:N/A (2014). Mouse . Read more (PubMed: 24676140) »
  • Végran F  et al. Association of p53 gene alterations with the expression of antiapoptotic survivin splice variants in breast cancer. Oncogene 26:290-7 (2007). Read more (PubMed: 16847456) »

See all 5 Publications for this product

Product Wall

DISCOUNT CODES: ab3729: xxxxx ab3731: xxxxx Ablaufdatum (jeweils): xxxx2012 Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab3729 und ab3731 in der Immunhistochemie an Paraffinschnitten (IHC-P) zu testen, annehmen möchten. Wi...

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Thank you for contacting Abcam regarding ab76424 and ab3731. I am sorry that you have been experiencing difficulties getting these antibodies to work in WB.  I am following up with the lab regarding reactivity of ab76424 with Delta Ex3.  Regarding a...

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Thank you for your email. We have not had any reports of a band being detected at approximately 35 kDa with ab3731. We do sell the blocking peptide for this antibody which should be helpful for you - it is ab13763. Interestingly, it is noted for ab4...

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Thank you for your enquiry. Ab3731 should detect a band of ~ 14 kDa. Please let me know if you have any additional questions or require additional assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"