Anti-Survivin抗体- C-terminal,prediluted (ab15527)

概述

  • 产品名称
    Anti-Survivin抗体- C-terminal,prediluted
    参阅全部 Survivin 一抗
  • 描述
    兔多克隆抗体to Survivin - C-terminal,prediluted
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to Human Survivin (C terminal).
    Database link: O15392

  • 阳性对照
    • IHC-P: Human stomach tissue.

性能

应用

Our Abpromise guarantee covers the use of ab15527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

靶标

  • 功能
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • 组织特异性
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • 序列相似性
    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • 发展阶段
    Expression is cell cycle-dependent and peaks at mitosis.
  • 结构域
    The BIR repeat is necessary and sufficient for HBXIP binding.
  • 翻译后修饰
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • 细胞定位
    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

图片

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for Survivin using ab15527 in immunohistochemical analysis.

文献

ab15527 has not yet been referenced specifically in any publications.

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