The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 2 µg/ml.
IHC-P: 1/20 (human) - 1/400 (rat).
WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Belongs to the SFTPD family. Contains 1 C-type lectin domain. Contains 1 collagen-like domain.
The N-terminus is blocked. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
Yadav AK et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One9:e108815 (2014).
Read more (PubMed: 25303045) »
Kasper M et al. Monoclonal antibodies to surfactant protein D: evaluation of immunoreactivity in normal rat lung and in a radiation-induced fibrosis model. Exp Lung Res21:577-88 (1995).
Read more (PubMed: 7588444) »