35 kDa pulmonary surfactant-associated protein antibody
Alveolar proteinosis protein antibody
Collectin 4 antibody
Pulmonary surfactant apoprotein antibody
Pulmonary surfactant-associated protein A1 antibody
SP A antibody
SP A1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A antibody (ab87674)
IHC image of Surfactant Protein A staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Surfactant Protein A antibody (ab87674)
Anti-Surfactant Protein A antibody (ab87674) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique
Exposure time : 8 minutesSurfactant Protein A contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.